Establishment of a simple, easy and useful new recombinant virus manufacture system using a homologous recombination phenomenon

研究了重叠结构域的长度、位置和同源性对同源重组效率的影响，以及使用常规和新一代SHIV的基因导入方法和基因导入细胞等条件，并阐明了最佳条件。通过这种方法，我成功地重排了各种CCR 5型HIV-1临床分离株的env区，并保持了传统和新一代SHIV的多样性作为骨架。此外，我成功地将该方法应用于登革病毒和蜱骨脑炎病毒的构建。

I examined the influence that the length of the overlap domain, a position, and homology give in homologous recombination efficiency, and also the conditions such as the gene introduction method and the gene introduction cells using the conventional and new generation SHIV, and clarified the optimum. By this way, I succeeded in rearranging the env region of the various kinds of CCR5 type HIV-1 clinical isolates with maintaining variety in conventional and new generation SHIV as a backbone. Furthermore, I succeeded in an application of the method to Dengue virus and Tick-bone encephalitis virus construction.