On the production, fusion, and function of pollen protoplasts

本研究对各种高等植物成熟花粉原生质体的酶法分离、培养融合及其细胞壁再生后的功能表达进行了研究。1.被子植物花粉原生质体的分离。100多种双子叶植物中有8种获得了原生质体，31种单子叶植物中有27种获得了原生质体。2.长花百合的花粉原生质体培养2天后放在柱头上。3.在百合原生质体融合过程中，细胞壁的再生、花粉管的伸长和生殖细胞的分裂都表现出正常的行为。花粉管数目一般为1个，管内营养核先于营养核。在不同物种之间的原生质体融合中，通常有一根花粉管从融合体中延伸出来。4.在花粉与中生原生质体的融合中，花粉的功能是在细胞壁再生后表达的。5.利用气动枪在长花百合的花粉中成功地表达了b-葡萄糖醛酸苷酶基因。

Enzymatic isolation of protoplasts from mature pollen in various species of higher plants, their culture and fusion and their functional expression after the regeneration of cell wall were studied.1.Isolation of protoplasts from pollen in Angiosperms. The protoplasts were obtained from 8 dicotyledon species within more than 100 species tried, whereas 27 within 31 species in monocotyleadons.2.In Lilium longiflorum, pollen protoplasts were cultured for 2 days and placed on the stigma. Pollen tubes elongated into their pistil through ovary, and resulted in the seed formation.3.In fusion of lily protoplasts, the regeneration of cell wall, elongation of pollen tubes and the division of generative cells showed normal behavior. The number of of pollen tubes was usally one, and vegetative nuclei preceeded in the tubes. In protoplast fusion between defferent species, usually one pollen tube elongated from the fusion body.4.In fusion between pollen and mesohyll protoplasts, the function as pollen was expressed after the regeneration of cell wall.5.A b-glucuronidase gene was successfully expressed in pollen of Lilium longiflorum using a pneumatic particle gun.