The stimulative effect of macrophages and the influence of cytokines on hepatocyte

脂多糖与库普弗细胞或多形核细胞之间的反应产物被证明对胆汁分泌和胆小管功能具有特征性影响。实验证实胆流量和胆汁酸分泌减少、溶酶体活化、线粒体紊乱和胆管形态改变。为了澄清上述现象，我们研究了脂多糖与巨噬细胞之间培养基中某一组分的单克隆抗体。获得的几种单克隆a抗体对该部分的功能没有明显的抗作用。这些抗体与已知的细胞因子il -1、IL-6、IL-8、IL-10、TNF、PAF之间存在相关性，无特异性相关性。也就是说，这种单克隆抗体对这些细胞因子没有特异性。然而，其中一种通过培养基的反应产物具有抗肝细胞毒性的作用。更多的这种抗体和抗原正在研究中。在体内观察门静脉给药实验。在体外实验中，将该抗体与市面上常见的细胞因子抗体在大鼠肝细胞中的毒性和调节作用进行了比较。该系列生产的单克隆抗体没有这种高度特异性的现象，因此大多数体内实验都采用了商品抗体。抗体预处理对总胆汁流量、胆汁酸浓度变化及胆小管形态变化均有显著影响，但对反应产物灌注后的后处理无影响。从活性产物激活肝细胞溶酶体酶和降低肝细胞线粒体atp酶的角度来看，抗体在“体外”实验中比在“体内”实验中有显著的使用现象。一种未知的单克隆抗体及其抗原的特性被认为是重要的，将在本研究中继续研究。少

Reactive products between lipopolysaccharide and Kupffer cells or polymorphonuclear cells were proven to have a characteristic effect on secretion of bile and on function of bile canaliculus. Experimentally, decrease of bile flow and bile acids secretion, lysosomal activations, mitochondrial disturbances and the morphological changes of bile canalidulus are confirmed. In order to clarify above phenomena, monoclonal antibodies for one fraction of culture media between lipopolysaccharide and macrophages were studied. None among several monoclonal a ntibodies gained had significant anti-effects for the functions of that fraction. The relationships between those antibodies and the well-known cytokines, i.e.IL-1, IL-6, IL-8, IL-10, TNF and PAF,were made sure and no-specific correlation was observed. Namely, this monoclonal antibody is not specific for those cytokines. Howerver, one of them has an anti-effect of hepatocyte cytotoxicity by reactive product from cultured media. In details abou … More t this antibody and antigen are in study. The administration experiment via the portal vein was observed in vivo. And also in vitro, the effect of this antibody had been compared to that of the commercial antibodies for well-known cytokines about cytotoxicity and modulationin rat hepatocytes. The highly specific phenomena could not be found in a monoclonal antibody produced in this series, therefore, the commercial antibodies were utilized in most of in vivo experiment. The pretreatments by those antibodies had significant effects of total bile flow, the changes of bile acid concentrations and the morphological changes of bile canaliculus but no effect is obseved in the posttreatment following the perfusion of reactive products.The significant phenomena using by antibodies were observed in "in vitro" experiment than in "in vivo", on the point of views, i.e.the activation of hepatocyte lysosomal enzymes and the decrease of hepatocyte mitochondrial ATPase by the reactive products. The characteristics of an unknown monoclonal antibody and its antigan is recognized to be important and will be continued to study in this research. Less