functional studies of genes selectively expressed in primate eocortical areas

首先，我们的目标是分析在灵长类新皮质区域选择性表达的基因的功能，并与啮齿动物中的基因进行比较。我们检测了大鼠接受视听多感觉任务时c-Fos的表达。我们发现，在暴露于任务时，c-Fos在V2L(外侧次级视觉区域)的表达特异性增强。利用先前建立的皮质盒方法，我们分析了大鼠新皮质中的三层特异性基因(Rorb、Er81、Nurr1)。这三个基因都表现出互斥和互补的表达模式，这表明啮齿类动物的区域能够被自动或客观地描述，尽管区域选择性表达不如灵长类动物显著。我们报告了SLIT1基因选择性地在猕猴新皮质的关联区表达。最后，我们建立了灵长类新皮质的基因操作系统。

First, we aimed to analyze the function of the genes selectively expressed in the primate neocortical areas in comparison with that in rodents. We examined the expression of c-Fos when rats were subjected to an audio-visual multisensory task. We found that specific enhancement of c-Fos expression in V2L (the lateral secondary visual area) upon being exposed to the task. Using previously developed cortical box method, we analyzed three layer specific genes (RORb, ER81, Nurr1) in the rat neocortex. Each of three genes showed a mutually exclusive and complimentary expression pattern, which demonstrated that the areas in rodents were able to be automatically or objectively described although the area selective expression was not as prominent as that in primates. We reported SLIT1 as a gene that was selectively expressed in the association areas of the macaque neocortex. Finally, we have established the gene manipulation system in the primate neocortex