PHOTODYNAMIC THERAPY OF BLADDER CANCER BY YAG-OPO LASER (BASIC STUDY)

利用5-氨基乙酰胆酸=5- ala在体内产生的光敏剂原卟啉- ix （Pp-IX）对移植于C3H/He小鼠背部的鳞状细胞癌（SCC）肿瘤具有亲和力，研究了新型半导体激光器（iLS-LD400模型，405 nm, 5 mW）的光动力学大诊断（PDMD）。结果发现，光谱学数据表明，经47 kHz、50 W、10 min超声照射后，组织中Pp-IX的荧光强度增强2倍。此外，还研究了光动力（PDT）和声动力（SDT）（47 kHz, 50 W， 10秒）联合治疗黑色素瘤培养细胞（HMF），使用声敏剂ATX-70、卟啉镓衍生物和新型激光器iLS-LD400 （405 nm, 5 mW, 10 min）。结果发现，联合治疗对坏死细胞死亡有促进作用，特别是在溶声前先进行光疗的情况下，其促进作用优于其他情况。综上所述，建立利用新型半导体激光器（iLS-LD400模型，IHI）对膀胱肿瘤合并5-ALA模型进行宏观诊断，并利用激光治疗上皮移行细胞癌的方法。

We investigated the photodynamic macrotic diagnosis (PDMD) of a cancer by a new semiconductor laser (iLS-LD400 model, 405 nm, 5 mW) using the photosensitizer protoporphyrin-IX (Pp-IX) generated in vivo from 5-aminolevulittic acid=5-ALA which has an affinity for the squamous cell carcinoma (SCC) tumor transplanted on the back of C3H/He mouse. In the result, it was found the specific affinity of the Pp-IX in the SCC tumor from the spectroscopic data that the Pp-IX fluorescent intensity in the tissue was enhanced 2 times by the ultrasound irradiation (47 kHz, 50 W, 10 min).Furthermore, it was also investigated the combination therapy of photodynamic (PDT) and sonodynamic (SDT)(47 kHz, 50 W, 10 sec) treatments of a melanoma cultivated cell (HMF) using a sonosensitizer ATX-70, gallium porphyrin derivative, and the new laser iLS-LD400 (405 nm, 5 mW, 10 min). In the results, its was found that the necrotic cell death was enhanced by the combination therapy, especially in the case of the first treatment of the photoirradiation before the sonolysis, the enhancing effect was better than the other case.In conclusion, it will be established to do the macrotic diagnosis the bladder tumor incorporated 5-ALA in the bladder model using a new semiconductor laser (iLS-LD400 model, IHI) and to treat the epithelium transitional cell carcinoma by the laser light.