Establishment of the Analytical System on a Fine Volume Synovial Fluid in Human Temporomandibular Joints

1)通过横断面研究探讨颞下颌关节盘病变患者直接抽吸滑液（SF）中硫酸角质素（KS）的浓度。55例女性患者（平均34.1岁，平均29岁）62个关节经MRI诊断为前移位（WR; 24个关节）或未复位（WOR; 24个关节）和骨关节炎（OA; 14个关节），KS浓度采用单克隆抗体（1-20 5D4）电化学发光免疫分析法（ECLIA）定量。在62个关节（100%）中，SF中可检测到KS。WR、WOR和OA组KS浓度的模式和范围分别为1779.4、617.22和387.75，280.32-39495.5、64-8740和126.48-3510.5 ng/ml，分别两组间的差异具有统计学意义（p<0.05）。2）在横断面研究中，研究椎间盘疾病患者在囊内冲洗期间恢复的样本中TMJ或具有可检测的白细胞介素6（IL-6）的样本的频率。14例女性患者（年龄16 ~ 60岁，平均37.0岁，众数26岁）的14个关节经MRI诊断为椎间盘前移位，未复位。在20个14 ml试管中各获得约10 ml回收样品，并进行时间序列采集。用真空冷冻干燥机将每份样品浓缩至约2 ml。采用酶联免疫吸附试验（ELISA）试剂盒，用亲和素-生物素扩增，两步夹心法测定IL-6的浓度。282例标本中有116例（41.1%）或12例（85.7%）关节标本IL-6阳性。关节中可检测到IL-6的样本频率范围为90.0%至0%（平均值41.4%，中位数40%）。3个检出率超过85%的关节与2个未检出的关节表现出不同的临床特征。

1) To investigate in a cross sectional study the concentrations of keratan sulfate (KS) in the direct aspirated synovial fluid (SF) in patients with TMJ disc dis-orders. Sixty-two joints of 55 female patients (mean 34.1, mode 29 years old) were diagnosed as an anterior displaced disc with (WR; 24 joints) or without reduction (WOR; 24 joints), and with osteoarthritis (OA; 14 joints) by MR imagery.KS concentrations was quantified by electrochemilumine-scence immunoassay (ECLIA) with a monoclonal antibody (1-20 5D4). In 62 joints (100%), KS in SF was detectable. The mode and ranges of KS concentrations in the WR, WOR and OA groups was 1779.4, 617.22 and 387.75, and 280.32-39495.5, 64-8740 and 126.48-3510.5 ng/ml., respectively. This difference among the groups was statistically significant (p<0.05).2) To investigate in a cross sectional study the frequencies of the TMJ or the sample with detectable Interleukin 6 (IL-6) in the recovered samples during the intra capsular irrigation in patients with disc disorders. Fourteen joints of 14 female patients (ranged 16 to 60, mean 37.0 years, mode 26 years) were diagnosed as an anterior displaced disc without reduction by MR imagery. The recovered samples were obtained approximately 10 ml each in the twenty 14ml-test tubes with the time-series collection. Each sample was concentrated into approximately 2 ml with vacuum freeze dryer. The concentration of IL-6 was quantified by enzyme-linked immuno-sorbent assay (ELISA) kit with the avidin-biotin amplified, two step sandwich method. In 12 joints (85.7%) or 116 samples of all 282samples(41.1%), IL-6 was detectable. The frequencies of samples with detectable IL-6 in the joint each were ranged from 90.0% to 0% (mean 41.4%, median 40%). Three joints with over 85% detectable frequency showed the different clinical feature from the undetectable 2 joints.