Antinucleic aid antibody gene therapy for rheumatoid Arthritis and adult T cell Leukemia

1.抗DNARNA杂交体抗体在细胞中的表达。我们首先获得了产生单克隆抗dma RNA杂交抗体的杂交瘤，制备了编码该抗体的VH和VL基因片段。将这两个片段结合后表达ScFv抗体，并导入载体在细胞中表达。抗scfv表达系统基因治疗预防烟草花叶病毒（TMV）感染的效果2 .引入抗dnarna杂交ScFv基因的烟草表达了ScFv mRNA，对TMV感染表现出明显的抗性。抗scfv表达系统基因治疗人成人T细胞白血病病毒（HTLV-1）感染阻断实验我们正在建立ScFv策略的有效性评估，以阻断HTLV-1在T细胞中的感染。

1.Expression of antibody against DNARNA hybrid in the cells.We first obtained hybridoma producing monoclonal anti-DMA RNA hybrid antibody and prepared the fragments of VH and VL gene encoding the antibody. Both fragments were bound for the expression of ScFv antibodies and introduced into vectors for the expression in the cells.2. Efficacy of gene therapy using anti-ScFv expression system for prevention of tabacco mosaic virus (TMV) infection.Tabacco which was introduced with anti-DNARNA hybrid ScFv genes, expressed ScFv mRNA and showed marked resistance to TMV infection.3. Blocking experiment of human adult T cell leukemia virus (HTLV-1) infection by gene therapy using anti-ScFv expression system.We are establishing for evaluation of efficacy of ScFv strategy in order to block HTLV-1 infection in T cells.