The pathological influence on ultrastructure of the red blood cell and the vascular system under long term circulation with continuous flow left heart bypass.

1.剪切应力作用下红细胞膜的超微结构变化我们研究了加载剪切应力下红细胞膜的超微结构变化。结果表明，连续暴露于低切应力下，红细胞膜带3密度增加，变形能力下降。结果表明存在导致细胞破碎的另一过程。低切应力下红细胞膜骨架呈现延迟性破坏.本研究的目的是探讨长时间连续流左心转流（LHB）下主动脉平滑肌细胞（SMC）在主动脉壁变薄的情况下血管重构过程中的作用。这些主动脉SMC保持收缩形成。然而，这些细胞合成多余的基质金属蛋白酶-2&9。SMC表现出合成-去分化形成。总之，长时间持续性动脉高压下主动脉壁变薄 ...更多信息 流动性LHB是由SMC过度合成基质金属蛋白酶引起的，是细胞外基质的血管重塑过程.长时间连续流LHB下的小动脉我们观察了长时间连续流LHB下肾脏动脉系统的形态学变化。长时间连续血流LHB后，SMC数量增加，常使传入小动脉增厚。长时间持续血流LHB可引起肾内小动脉平滑肌细胞增生.长时间连续流LHB对脑组织的影响我们研究了长时间连续流LHB对脑组织病理学的影响。持续血流组大脑中动脉壁厚度与正常山羊无明显差异。血管平滑肌细胞的含量和性质也保持在正常范围内。长时间连续流LHB下的脑组织病理改变和血管系统均在正常范围内。少

1. Ultrastructural alterations in red blood cell (RBC) membranes exposed to shear stressWe investigated the ultrastructural alterations of RBC membrane with loaded shear stress. The result with continuous exposure to low shear stress showed an increase in band 3 density, and a decrease in the deformability of the RBC membrane. The results indicate the presence of another process leading to cell-fragmentation. The RBC membrane-skeleton with low shear stress shows delayed destruction.2. Aortic smooth muscle cells (SMC) under prolonged continuous flow left heart bypass (LHB).The goal of this study was to determine the roles of SMC in the vascular remodeling process in cases demonstrating aortic wall thinness under prolonged continuous flow LHB. These aortic SMC maintained contractile formation. These cells, however, synthesized redundant matrix metalloproteinase-2 & 9. The SMC demonstrated synthetic-dedifferentiated formation. In conclusion, aortic wall thinness under prolonged continuous … More flow LHB is caused by over-synthesis of matrix metalloproteinase in SMC, and these refers the vascular remodeling process of the extracellular matrix.3. The small artery under prolonged continuous flow LHBWe investigated morphological changes of the arterial systems in the kidneys under prolonged continuous flow LHB. The afferent arterioles were frequently thickened by an increase in the number of SMC after prolonged continuous flow LHB. Prolonged continuous flow LHB causes proliferation of SMC in the afferent arterioles in the kidneys.4. The brain under prolonged continuous flow LHBWe investigated the influence on the cerebral pathological findings under prolonged continuous flow LHB. The wall thickness of middle cerebral artery of the continuous flow group was not different from that of normal goats. The value of the vascular SMC contents and the properties of vascular SMC were also kept within normal range. The cerebral pathological findings and vascular systems under prolonged continuous flow LHB were within normal range. Less