Role of mast cells in allergic rhinitis and nasal polyps : Gene chip Analysis

本研究的目的是探讨肥大细胞在鼻变态反应和鼻息肉中的作用。首先，我们从变应性鼻炎患者的鼻甲和鼻息肉（包括阿司匹林敏感的鼻息肉）中分离CD 34+祖细胞。然后将这些细胞培养以分离MC和MCTC肥大细胞。然后我们通过FcεRI和TLR刺激这些肥大细胞。我们发现MCTC肥大细胞表达CCR 3和CCR 5，表达GMCSF、IL-4、IL-5、IL-6、IL-8、IL-13、IL=16和eotaxin。当这些细胞通过TLR刺激时，MCTC细胞表达GMCSF、IL-4、IL-5、IL-6、IL-8、IL-10、IL-13、IL-16和RANTES。使用这种培养的肥大细胞模型，我们发现RANTES是肥大细胞迁移到上皮的重要趋化因子。我们还发现，干细胞因子（SCF）上调肥大细胞糜蛋白酶的表达。流式细胞仪分选Cd 34+细胞，并与SCF IL-6共同培养于96孔板的甲基纤维素培养基中，形成集落培养。然后从10^7个呼叫中提取mRNA，通过基因芯片分析进一步分析其特征

The objective of our research was to elucidate the role of mast cells in nasal allergy as well as nasal polyps, Firstly, we isolated CD34+progenitor cells from nasal turbinates of allergic rhinitis patients, and nasal polyps including those with aspirin sensitivity. Then we cultured these cells to isolate MC and MCTC mast cells. We then stimulated these mast cells via the FcεRI and TLR. We found that MCTC mast cells express CCR3 and CCR5 and express GMCSF, IL-4,IL-5,IL-6,IL-8,IL-13,IL=16 and eotaxin. When these cells were stimulated via TLR MCTC cells express GMCSF, IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,IL-16 and RANTES.It is known that there is an increase in mast cells in the nasal epithelium of allergic rhinitics. Using this cultured mast cell model, we found that RANTES is an important chemotactic factor for mast cell migration into the epithelium. We also found that Stem cell factor (SCF) upregulates chymase expression in mast cells. Furthermore, we FACS sorted Cd34+ progenitor cells and cultured them with SCF IL-6 in 96well plate in methyl cellulose culture to form a colony culture. Then from 10^7 calls we extractedmRNA to further analyze their characteristics by Gene chip analysis