Global regulation of genome expression : Single cell analysis of environmental stress response

模式细菌大肠杆菌能够在自然界的各种压力条件下生存，通过形成细菌群落，其中每个细胞发挥不同的生理作用。为了寻找它们各自的作用，使用新构建的细胞芯片开发了单活细胞观察系统，并分析了单个细胞的转录。为了进一步了解基因组转录的调控机制，我们还对大肠杆菌中300多个转录因子的调控作用进行了系统分析。

The model bacterium, Escherichia coli, is able to survive under various stressful conditions in nature by forming the bacterial community, in which each cell plays a different physiological role. For search of the role each plays, single-live cell observation systems were developed using newly constructed cell chips, and transcription in individual cells was analyzed. Towards understanding the regulation mechanism of genome transcription, we also performed the systematic analysis of regulatory roles of a to tal of 300 transcription factors from E. coli.