Surface modification of islets for increasing graft survival rate

再生医学和组织工程开辟了新的治疗领域。较成功的尝试之一是移植朗格汉斯岛（胰岛），它可以产生和释放胰岛素，作为1型糖尿病患者的治疗方法。然而，当植入活体组织时，胰岛会引起各种不良的生物反应，包括血液凝固、补体激活、炎症反应和免疫反应。我们开发了一种新的方法，将蛋白质和细胞固定在胰岛表面，以增加它们与宿主环境的相容性。胰岛表面被ssdna - peg -脂质修饰。蛋白质或细胞被与ssDNA互补的ssDNA修饰。通过ssDNA和ssDNA的杂交，可以将蛋白质或细胞固定在细胞上。

Regenerative medicine and tissue engineering have opened new therapeutic domains. One of the more successful endeavors has been the transplantation of islets of Langerhans(islets), which produce and release insulin, as a treatment for patients with type 1 diabetes. However, when implanted into living tissue, islets can induce various undesirable biological responses, including blood coagulation, complement activation, inflammatory reactions, and immune reactions. We developed a new method in whichproteins and cells are immobilized on the islet surface to increase their compatibility with a host environment. Islet surface is modified with the ssDNA-PEG-lipid. Proteins or cells are modified with a ssDNA' that is complementary to the ssDNA. The proteins or the cells can be immobilized on the cell through hybridization between the ssDNA and ssDNA'.