Insulin releasing cells derived from embryonic stem cells and new semipermeable membranes for bioartificial pancreas.

来自胚胎干细胞的胰岛素释放细胞和用于生物人工胰腺的新型半透膜。

基本信息

  • 批准号:
    14207048
  • 负责人:
  • 金额:
    $ 34.36万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
  • 财政年份:
    2002
  • 资助国家:
    日本
  • 起止时间:
    2002 至 2005
  • 项目状态:
    已结题

项目摘要

Dopaminergic Neurons Derived from ES Cells : Neural inducing factors (NIFs) produced by washing PA6 cells were collected. ES cells enclosed in semipermeable membranes could be efficiently differentiated to dopaminergic neurons using medium containing NIFs. β-tubulin type III positive cells and tyrosine hydroxylase positive cells were clearly observed in ES cell progenies after 16 days culture, and dopamine release were seen when cells were exposed to 56 mM KCl for 15 mm through deporization of neurons. Differentiation of ES cells in semipermeable membranes is one of crucially important procedures when cell therapy of Parkinson's disease will be realized.Insulin Releasing Cells Derived from ES Cells : Insulin releasing ES cell progeny was derived in culture media with/without fetal calf serum by the 4 step procedure. Cells of which cytoplasm was stained by the anti-insulin antibody and by anti-C-peptide antibodies were obtained. Those cells can be subculture 9 times. The results suggest that a sufficient number of insulin releasing cells could be obtained from ES cells for the cell transplantation therapy of diabetic patients.Membrane for Immunoisolation : An amino groups-terminated poly (ethylene glycol)-conjugated phospholipids (PEG-lipids, Mw : 5000) spontaneously formed a thin layer on cells, when they were added to cells suspension. The layer-by-layer membrane could be further formed on the PEG-lipid layer through the polyion complex formation between amino groups at the end of PEG chains, sodium alginate and poly-L-lysine. Cells could be microencapsulated by this method without volume increase.
ES细胞衍生多巴胺能神经元:收集PA6细胞洗涤后产生的神经诱导因子(Neural inducing factors, nif)。在含有nif的培养基中,半透膜包裹的胚胎干细胞可以有效地分化为多巴胺能神经元。培养16天后,胚胎干细胞子代中明显可见β-微管蛋白III型阳性细胞和酪氨酸羟化酶阳性细胞,当细胞暴露于56 mM KCl中15 mM时,通过神经元脱porization可以观察到多巴胺的释放。胚胎干细胞在半透膜中的分化是实现帕金森病细胞治疗的关键步骤之一。从胚胎干细胞中获得胰岛素释放细胞:通过4步程序在含/不含胎牛血清的培养基中获得释放胰岛素的胚胎干细胞后代。获得细胞质经抗胰岛素抗体和抗c肽抗体染色的细胞。这些细胞可以传代培养9次。提示胚胎干细胞可获得足够数量的胰岛素释放细胞,用于糖尿病患者的细胞移植治疗。免疫隔离膜:端氨基聚乙二醇偶联磷脂(PEG-lipids,分子量:5000)加入细胞悬浮液时,在细胞上自发形成薄层。通过聚乙二醇链末端的氨基与海藻酸钠和聚l -赖氨酸形成多离子络合物,进一步在聚乙二醇脂质层上形成一层一层的膜。该方法可以在不增加细胞体积的情况下实现细胞微囊化。

项目成果

期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Murakami Y.: "Dextran sulface as a material for the preparation of a membrane for immunoisolation."J Biomater Sci Polym Ed.. 14・9. 875-885 (2003)
Murakami Y.:“作为制备免疫隔离膜的材料的葡聚糖硫酸酯。”J Biomater Sci Polym Ed.. 14・9 (2003)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Bioartificial pancreas research in Japan.
日本的生物人工胰腺研究。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Takamizawa;A.;Fujimaki;S.;Sunner;J.;et al.;Iwata H.
  • 通讯作者:
    Iwata H.
Deposition of complement protein C3b on mixed self-assembled monolayers carrying surface hydroxyl and methyl groups studied by surface plasmon resonance
Iwata H.: "Bioartificial pancreas research in Japan."Artif Organs. 28・1. 45-52 (2004)
Iwata H.:“日本的生物人工胰腺研究”。Artif Organs 28・1(2004)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Induction dopamine releasing cells from mouse embryonic stem cells and their long-term culture.
来自小鼠胚胎干细胞及其长期培养物的诱导多巴胺释放细胞。
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IWATA Hiroo其他文献

IWATA Hiroo的其他文献

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{{ truncateString('IWATA Hiroo', 18)}}的其他基金

Transplantation of islets in immune tolerance site under skin induced by bFGF or SEK-1005
bFGF 或 SEK-1005 诱导的皮下免疫耐受部位移植胰岛
  • 批准号:
    26670577
  • 财政年份:
    2014
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Evacuation Simulator Using Locomotion Interface
使用运动接口的疏散模拟器
  • 批准号:
    25240030
  • 财政年份:
    2013
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Transplantation of co-aggregates of islet cells and immune suppressor cells
胰岛细胞和免疫抑制细胞共聚集体的移植
  • 批准号:
    24240078
  • 财政年份:
    2012
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Study of cell-cell interaction: Cell manipulation method for tissue regeneration
细胞与细胞相互作用的研究:组织再生的细胞操作方法
  • 批准号:
    23650257
  • 财政年份:
    2011
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Surface modification of islets for increasing graft survival rate
胰岛表面修饰以提高移植物成活率
  • 批准号:
    21240051
  • 财政年份:
    2009
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of Generic Technology of Locomotion Interface
运动接口通用技术的发展
  • 批准号:
    21240006
  • 财政年份:
    2009
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of 3D Infinite Walkthrough Space and its Evaluation
3D无限漫游空间的开发及其评价
  • 批准号:
    18300040
  • 财政年份:
    2006
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Encapsulation of Islets with Amphiphilic Polymers for Immuno-isolation
用两亲性聚合物封装胰岛以进行免疫隔离
  • 批准号:
    18200034
  • 财政年份:
    2006
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Development of an Omni-directional Virtual Walk-space and its Evaluation
全方位虚拟步行空间的开发及其评价
  • 批准号:
    14350126
  • 财政年份:
    2002
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Spatial Recognition Performance Tests Using Omni-directional Infinite Surface
全向无限曲面空间识别性能测试
  • 批准号:
    11650261
  • 财政年份:
    1999
  • 资助金额:
    $ 34.36万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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