Examination of Bioartifichial Pancreac for future clinical use.

生物人工胰腺的设计是将胰岛组织和一种选择性渗透膜结合在一起，这种膜将胰岛与受体的免疫系统隔离开来。本研究旨在探讨琼脂糖微囊化同种异体胰岛和异种胰岛作为生物人工胰腺的可行性。同种异体移植：以5只全胰切除的糖尿病犬为受体，在无免疫抑制的情况下，观察微囊化胰岛的功能效果。将一定量的来自远系杂种供体的微囊化胰岛通过导管移植到胰腺切除的受体的网膜组织中。5例受者中有3例在无外源性胰岛素的情况下，平均空腹血糖分别控制在120 mg/dl以下28、42、49 d，分别接受4.3 × 103、7.3 × 103和1.0 × 104（IE/kg）微囊化胰岛。我们已经证明，由琼脂糖-聚苯乙烯磺酸混合物制成的微胶囊在啮齿动物模型中有效地延长了异种胰岛的存活期。我们为奈良医科大学研究小组提供琼脂糖和聚苯乙烯磺酸。他们将我们的微胶囊应用于猪胰岛移植给犬受体。受体的血糖正常期分别为14、42、89和99 d。总之，本研究表明，微囊化胰岛可以在大型糖尿病动物中发挥作用，从而在不需要免疫抑制的情况下长期独立于外源性胰岛素治疗。

The bioartificial pancreas was designed to incorporate islet tissues and a selectively permeable membrane that isolates islets from the immune system of the recipient. The aim of this study is to examine the possibility of the agarose microcapsule containing allo-islets and xeno-islet as a bioartificial pancreas in canine model.Allograft : Functional efficacy of the microencapsulated islets was determined using five totally-pancreatectomized diabetic dogs as recipients without immunosuppression. Defined quantity of microencapsulated islets from outbred mongrel donors were grafted through the catheter into omental tissue of the pancreatectomized recipients. In three of five recipients, the average fasting glucose values were controlled under 120 mg/dl for 28,42,49 days without exogenous insulin, which received totally 4.3*103,7.3*103 and 1.0*104 (IE/kg) of microencapsulated islets, respectively.Xnoe-graft : We have demonstrated that the microcapsule made of an agarose-polystirene sulfonic acid mixture effectively prolonged xeno-islet survival period in rodent models. We supply the agarose and polystirene sulfonic acid to the research group of Nara medical school. They applied our microcapsule to transplantation of porcine islet to canine recipients. The normoglycemic periods of the recipients are 14,42,89, and 99 days.In conclusion, the present study indicates that the microencapsulated islets can function in large diabetic animals, resulting in the independence of exogenous insulin therapy for prolonged periods without the need for immunosuppression.