Mechanical Stress-Loading on Cellular Responses of Vascular Cell Types

本实验研究了细胞在机械应力场作用下的反应，包括体外贴壁细胞在二维基质上的定向和伸长以及体内血管壁在连续加载的机械应力场作用下的形态发生和组织形成，贴壁细胞在连续循环扩张时，随着机械应力加载时间的延长，细胞的定向与扩张方向平行。另一方面，使用血管细胞类型（内皮细胞; EC，平滑肌细胞; SMC和成纤维细胞; FC）制备的混合血管移植物在植入后进行自我重塑。内皮细胞高度伸长的流动方向，而SMC是周向取向。细胞分泌的细胞外基质（ECM）聚合物经历自组装和组织形成纤维形成与时间。这些ECM聚合物包括胶原蛋白和弹性蛋白。前者构成了高度环向取向的纤维网络，后者则形成了层状蜂窝结构。因此，鞍和大分子定向及时进行，以恢复新生动脉血管的重要结构和功能。

This study was conducted to study the responses of cells exposed to mechanical stress fild, which include orientation and elongation of adhered cells on 2-dimensional substrate in vitro and morphogenesis and tissue formation of vascular wall in vivo under continuously loaded mechanical stress fields.Upon continuous cyclic distension of adhered cells, cells oriented parallel to the direction of distension with loading time of mechanical stress. On the other hand, hybrid vascular grafts prepared using vascular cell types (endothelial cell ; EC,smooth muscle cell ; SMC and fibroblast ; FC) underwent to remodel themselves upon implantation. ECs highly elongated to the direction of flow, whereas SMCs are circumferentially oriented. Extracellular matrix (ECM) polymers secreted by cells underwent self-assembling and organized to form fiber formation with time. These ECM polymers include collagen and elastin. The former constructed a network of fiber highly oriented circumfererfially, and th later formed a layred honey-comb structure. Thus, sellular and macromolecular orientation proceeded in time to restore vital structure and function of neoarterial vessels.