Multiphasic studies on mechamistic asrects of Biocompatiblity of Materials used far Cardiouascular Devices

本研究旨在评估用于心血管人工装置的血液接触面的双相容性。由于涉及血栓形成的机体防御机制的复杂性，我们进行了一项多相研究，以确定血液/材料界面生物反应的性质和程度。新建立的接触活化度定量测定方法明确区分促凝表面和内表面。利用纯化凝血因子和补体因子重组系统的研究表明，这两种生物系统激活初始状态下的分子事件在分子水平上得到了清楚的理解。另一方面，合成的四肽（Arg-Gly-Asp-Ser）以剂量依赖的方式抑制了吸附的极化蛋白介导的血小板粘附，该四肽最近被发现是纤维蛋白原、纤维连接蛋白和玻璃体连接蛋白等粘附蛋白的共同粘附位点。这些研究从每个生物系统的表面反应性-生物活化输入一个一般的“表面性质-生物关系”，这将作为生物相容性表面分子设计的逻辑基础。L作为分子设计的逻辑基础

This study aims to assess the bicompatibility of blood-contacting surfaces used for cardiovascular artificial devices. Due to the complex nature of body defense mechanisms involving in the thrombus formation, a multiphasic study was conduced to determine the nature and magnitude of the biological responses at blood/material interfaces. Newly-developed quantitative assay method determining the degree of contact activation clearly differentiated the coagulation-promoting surfaces and innert surfaces. The studies using reconstituted systems using purified coagulation and complement factors showed that the molecular events at the initial states of activation of both biological systems were clearly understood at molecular level. On the other hand, the platelet adhesion mediated via adsorbed polasma proteins were inhibited in a dose-dependent manner by using synthetic tetrapeptida (Arg-Gly-Asp-Ser) which was recently identified as the common attachment site of adhesive proteins such as fibrinogen, fibronectin and vitronectin. These studies compiled from the surface reactivity-biological activation of each biological systems input a general "Surface Property-Biological Relationshuip", which will serve as a logical basis of the molecular design of biocompatible surfaces.l serve as a logical basis of the molecular design