Molecular and Morphological Studies on Mechanism for Maternal Inheritance of Chloroplast Genomes in Chlamydomonas reinhardtii.

在莱茵衣藻中，雌配子和雄配子两性融合后，雄配子来源的叶绿体DNA优先在受精卵中消失，但这种优先融合的机制仍有待建立。我们先前已经证明，当渗透的配子在有钙离子存在的情况下用衣藻核酸酶C孵化时，雄性来源的叶绿体DNA优先被破坏，而雌性配子则明显完好无损。然后，在本工作中，我们研究了雌雄配子在分子和形态上的差异。目的：1.制备针对幼龄受精卵细胞表面的单抗，以阐明两者之间的差异。制备保持完整形态的叶绿体类核，以研究它们的差异是否与细胞器DNA结合蛋白的差异有关。DNA结合蛋白经复性后可在含有DNA的SDS-凝胶中检测到。从特异性和敏感性两个方面讨论了DAPI替代EtBR的有效性。

In Chlamydomonas reinhardtii, the chloroplast DNA of male gamete origin preferentially disappears in the zygote after female and male gametes fuse sexully with each other, but the mechanism underlying this preferential dest-ruction remains to be established.We have previously shown that when the permeabilized gametes were incuba-ted with Chlamydomonas nuclease C in the presence of Ca^<2+>, chloroplast DNA from the male origin was preferentially destroyed, leaving female one apparently intact. Then, in the present work, we studied on the molecular and morpholo-gical difference between female and male gametes.1. Monoclonal antibody directed to cell surface of young zygotes was pre-pared to clarify the difference between them.2. Chloroplast nucleoids retaining the intact morphology were prepared to investigate whether their difference was due to that of DNA-binding proteins bound to organella DNA.3. The DNA-binding proteins could be detectable in SDS-gel containing DNA after their renaturations. Usefulness of DAPI instead of EtBr was discussed in connection of specificity and sensitivity.