Microcirculatory Disturbances in Cerebral Ischemia ; Analysis of Pathophysiology and Therapeutic Approach.

1. 局灶性脑缺血微循环障碍的进展。研究了阻断大鼠大脑中动脉（MCA）和沙鼠后交通动脉（P-com）后脑区域血流（rCBF）和血浆灌注微血管长度的变化。数据显示，动脉闭塞后几分钟内rCBF明显下降，30分钟或1小时后出现微血管血浆灌注紊乱，并在随后几小时内进一步恶化。微血管恶化的治疗方法。为了研究组织型纤溶酶原激活剂（tPA）和Cu， zn -超氧化物歧化酶（SOD）对脑缺血微血管恶化的影响，将重组人tPA和SOD持续应用于大鼠MCA闭塞模型。结果表明，静脉应用这些酶能显著改善微血管血浆循环。高血压大鼠微循环研究。测量了卒中易感自发性高血压大鼠（SHRSP）和年龄匹配的Wistar Kyoto大鼠单位体积微血管长度。数据显示，在SHRSP中，大脑多个区域的微血管长度减少。每日给予弹性蛋白酶制剂可改善SHRSP微血管血浆灌注。

1. Progression of Microcirculatory Disturbance in Focal Cerebral Ischemia.Changes in the regional cerebral blood flow (rCBF) and the length of plasma-perfused microvessels were studied after occlusion of the middle cerebral artery (MCA) in rats or the posterior communicating artery (P-com) in gerbils. The data demonstrated that the rCBF decreased markedly within several minutes after arterial occlusion, while the disturbances in microvascular plasma perfusion appeared 30 min or 1 hour later which further deteriorated for the following several hours.2. Therapeutic approach to microvascular deterioration.To investigate the effects of tissue-type plasminogen activator (tPA) and Cu, Zn-superoxide dismutase (SOD) on the microvascular deterioration during cerebral ischemia, recombinant human tPA and SOD were continuously applied in rat MCA occlusion model. The results indicated that intravenous application of those enzymes significantly improved the microvascular plasma circulation.3. Microcirculation in Hypertensive Rats.The microvascular length per unit volume was measured in the strokeprone spontaneously hypertensive Rats (SHRSP) and the age-matched Wistar Kyoto rats. The data demonstarted that the microvascular length was decreased in several areas of the brain in SHRSP. Daily administrattion of elastase prepartion improved the microvascular plasma perfusion in SHRSP.