Chemosensitivity test based on the intracellular level of free calcium

本文研究了顺铂、丝裂霉素C和阿霉素对小鼠肉瘤S-180细胞内游离钙离子浓度的影响。用荧光指示剂Fura-2测定[Ca^&lt；2+]i。在细胞体外孵育的前24小时，在药物存在的情况下，[Ca~(2+)]i低于对照细胞。孵育24小时后，细胞存活率降至对照组的50%时，[Ca~(2+)]i开始升高，最后降至10%，并超过1微米。[Ca^&lt；2+]i的升高与细胞活力的丧失是相互作用的。改变培养液中的Ca~(2+)浓度不改变结果。这些发现表明，在抗癌药物存在的情况下，随着这种离子从内部存储中释放出来，存在着游离Ca^lt；2+&gt；的积聚，细胞死亡伴随着细胞内钙离子动态平衡的破坏。

Alterations in concentrations of intracellular free calcium([Ca^<2+>]i) were examined in mouse sarcoma S-180 cells exposed to cisplatin, mitomycin C and adriamycin, as related to cell viability. The [Ca^<2+>]i was measured using the fluorescent Ca^<2+>indicator, fura-2. During the first 24 hr of incubation of the cells in vitro, in the presence of the drugs, the [Ca^<2+>]i was lower than in the control cells. After 24 hr of incubation, the [Ca^<2+>]i began to increase at a time when the cell viability was reduced to 50% of the controls, and finally exceeded 1 muM with reduction to 10%. The increase in [Ca^<2+>]i was reciprocal to the loss of the cell viability. Changed in Ca^<2+> concentrations in the medium did not alter the results. The findings suggest that in the presence of anticancer drugs there is an accumulation of free Ca^<2+> with a release of this ion from internal stores and that cell death follows disruption in the homeostasis of intracellular Ca^<2+>.