Physiological Research of Photoperiodic nesporse of insect using laser micro-beam.

研制了一套紫外光激光微束照射昆虫组织器官并检测其对昆虫光周期反应的影响的装置。首先，我尝试用激光微束照射到家蚕的食道下神经节(SG)，其中有激素效应系统。从滞育产卵者的蛹头上取出脑(BR)-SG复合体，放在玻璃板上。通过观察神经节表面产生的一些小气泡，证实了组织的损伤。当激光照射到SG中神经分泌细胞的位置时，滞育率为0%，而不照射的对照为100%，这表明这种激光治疗是一种非常有效的微治疗技术。关于家蚕对脑的光照，目前只有初步的结果，但原大脑神经节的一些部分的破坏表明，该区域可能存在一个中枢调控部位。

An apparatus which is devised to illuminate ultraviolet laser- microbeam to insect tissue and organ, and to examine its effect on the photoperiodic response of insects, was constructed. At first I tried an illumination of the laser-microbeam to subeophageal ganglion(SG) of the silkworm, in which the hormonal effector system is located. Brain(Br)-SG complex was extirpated from the head of pupae of diapause-egg producers, and setted on a glass- plate. The damage of the tissue was confirmed by an observation of some small bubbles which produced on the surface of the ganglions. When the beam was illuminated on the location of neurosecretory cells in SG the diapause percentage became 0%, while the control without illumination was 100%, it means that this laser-treatment was very effective as a micro-treatment technique. Regarding the illumination to brain of the silkworm, only preliminary results were obtained, but the destory of the some parts of protocerebral ganglion suggests that a central regulatory site might be located in this area.