Development of Small-Diameter Artificial Graff via Multifaceted Appproaches

在心血管手术中，小口径人工移植物的发展是人们期待已久的。早期植入时最小血栓形成，随后快速的新动脉再生和良好控制的形态发生对于病变血管的重要替代至关重要。为此，我们采取了多方面的方法，包括用准分子激光定向控制微孔隙度的支架设计，以提供顺应性匹配以及跨壁组织长入，基质设计和细胞因子固定，这两种方法都旨在增强内皮化和新动脉再生，以及细胞工程，提供具有三层分层结构的混合管状组织，仿造天然血管的分层。一旦这些学科被完全制定成设计移植物，这种移植物就会在体内进行自我重塑，产生与自然血管在结构和功能上相似的血管组织。

The development of small caliber artificial grafts has been long awaited in cardiovascular surgey. Minimal thrombus formation in an early implantation and rapid neoarterial regeneration and well-controlled morphogenesis in the following period are essential for vital replacement with diseased vessels. To this esd, we have taken multi-faceted approaches including scaffold design with excimer laser-directed controlled microporosity to provide compliance matching as well as transmural tissue ingrowth, metrix design and cytokine immobilization, both of which are designed to enhance endothelialization and neoarterial regeneration, and cellular engineering providing a hybrid tubular tissue with three-layred hierarchical structure biomimicking to the layring of natural vessels. Onece these disciplines were fully formulated into a design graft, such grafts undergo to remoldel themselves in vivo, producing a vascular tissues resembling that of natural vessel in terms of structure and function.