Searth fer adhesive molecule between plasma membrane and cell wall

细胞壁和质膜之间的关系在植物中被认为是非常重要的。细胞壁可能在执行各种生理现象中起着重要作用。在这些现象中，预测了通过质膜连接细胞内外部分的特殊蛋白质。在这项工作中，我们试图找到那些类似于整合素(或整合素基因)的蛋白质或它们的基因作为细胞外基质蛋白的受体。从一家公司购买的RGD肽被发现对破坏皮层微管以及诱导蕨类植物Adiantum Protonem细胞的细胞核和叶绿体形态变化非常有效。然而，从其他公司获得的多肽是无效的。原因是冰醋酸与RGD多肽混合，用于RGD多肽的提纯。醋酸对微管、细胞核和叶绿体形态变化的影响实验证实了这一结果。到目前为止，我们还没有从形态角度上发现RGD多肽对蕨类原丝体的发育过程有任何影响。虽然我们可以获得几个扩增片段，但到目前为止测序的所有标记都不是整合素相关蛋白。目前，我们还不能证明Adiantum中存在与整合素相关的蛋白质或基因。由于目前还没有关于植物整合素或整合素相关蛋白的确凿数据，因此植物可能使用其他蛋白质来连接细胞的内部和外部。

The ralationship between cell wall and plasma membrane is thought to be very important in plants. Cell wall may play an important role to perform various physiological phenomena. In these phenomena, special proteins onnecting outer and inner parts of the cells through the plasma membrane are predicted. In this work we tried to find those proteins or their genes resembling to integrin (or integrine gene) as a receptor for extracellular matrix proteins.RGD peptide purchased from a company was found to be very effective for destruction of cortical microtubules, as well as for induction of morphological changes of nuclei and chloroplasts in fern Adiantum protonemal cells. However the peptide obtained from other company was not effective. The reason was caused by acetic acid mixed with RGD peptide which was used for prification process of the RGD peptide. The results were confirmed by experiments of the effect of acetic acid on the morphological changes of microtubules, nuclei and chloroplasts. So far we could not find any influence of RGD peptide on the developmental processes of fern protonema in the morphological point of view.Newt, we tried to search for integrin-related genes in Adiantum by RT-PCR,using degenerated primers constructed from the consensus sequences of mammalian integrin genes. Although we could obtain several amprified fragments, all flagments so far sequenced were not integrin-related proteins. At this moment, we could not prove the existance of integrin-related proteins or genes in Adiantum. Since there is not conclusive data of integrin or integrin-related proteins from plants, it is plausible that plants use other proteins for connecting inner and ourter parts of cells.