A new culture system for studying in vitro human dental pulp repair

在这项研究中，我们调查了拔牙后立即钻孔和培养3天到1个月的人牙厚片的行为。结果表明，在体外，空洞下受损的牙髓能够形成一些与组织愈合相关的典型方面，表现为细胞增殖、新生血管和损伤区域附近存在功能立方细胞。培养30天后，可见细长的纺锤形细胞沿相关牙本质壁的边缘排列，而健康区域下功能良好的成牙本质细胞保存完好。组织修复使我们相信，这样的培养模型将是一个有用的系统，以测试调节牙髓修复的因素。

In this study, we investigated the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation, neovascularization, and the presence of functional cuboidal cells close to the injured area. After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. Tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.