Molecular biological and cytochemical study on the mechanisms of expression of steroid hormone synthesizing enzymes.

(1)获得3β和17β-羟基类固醇脱氢酶多克隆抗体。适用于免疫组织化学。(2)采用免疫组化和Western blotting方法检测大鼠胃壁细胞和垂体中间叶细胞17α-羟化酶/C17-20裂解酶。(3)免疫组织化学检测大鼠嗅球中芳香化酶、17β-羟基类固醇脱氢酶和5α-还原酶。(4)地塞米松通过激活糖皮质激素受体增强血清剥夺诱导的大鼠C6胶质瘤细胞坏死死亡。无血清条件下C6胶质瘤细胞胞质中出现5α-还原酶免疫反应性，可能不是由于5α-还原酶基因表达的改变。(5)肝型前列腺素F （PGF）合成酶免疫细胞化学定位于大鼠肝窦内皮细胞。(6)在大鼠脊髓中，肺型PGF合酶免疫定位于神经细胞和血管内皮细胞的树突，肝型PGF合酶免疫定位于血管内皮细胞和血管内皮细胞。(7)细胞色素b5对17α-羟化酶/C17-20裂解酶的功能起着重要的控制作用，但我们无法建立良好的抗细胞色素b5抗体。

(1) We got polyclonal antibodies against 3β-and 17β-hydroxysteroid dehydrogenases. They are applicable for immunohistochemistry.(2) 17α-Hydroxylase/C17-20 lyase was detected in rat gastric parietal cells and pituitary intermediate lobe cells by means of immunohistochemistry and Western blotting.(3) Aromatase, 17β-hydroxysteroid dehydrogenase and 5α-reductase were immunohistochemically detected in rat olfactory bulb.(4) Dexamethasone enhances serum deprivation-induced necrotic death of rat C6 glioma cells through activation of glucocorticoid receptors. The appearance of 5α-reductase immunoreactivity in the cytoplasm of C6 glioma cells under serum-free conditions was probably not due to changes in 5α-reductase gene expression.(5) Liver type prostaglandin F (PGF) synthase was immunocytochemically localized in the sinusoidal endothelial cells of rat liver.(6) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites of nerve cells and vascular endothelial cells, and liver type one in the tanycytes and vascular endothelial cells.(7) We could not set good antibody against cytochrome b5 which plays important roles in the contorl of function of 17α-hydroxylase/C17-20 lyase.