AN ATTEMPT TO INTRODUCE GLYCOBIOLOGICAL OR BIOCHEMICAL TECHNIQUES INTO FORENSICPATHOLOGICAL DIAGNOSIS

凝集素组织化学技术清楚地揭示了来自脑部疾病患者的人脑中存在老年斑、神经原纤维缠结、淀粉体和三种类型的碳水化合物沉积，即球形、血管状和层状。在精神分裂症患者海马结构齿状回分子层检测到球形沉积物。在痴呆、唐氏综合征、脑水肿等脑障碍患者的大脑和小脑白质区均可见苏木精伊红染色呈无定形的血管型和层状型。从人肝细胞质中纯化二肽基肽酶III （DPIII）和丙氨酰氨基肽酶（AP），并制备了针对这两种酶的抗体。抗AP抗体与神经元和胶质细胞发生组织化学反应，可对上述无定形沉积进行染色。本项目新发现的沉积可能是脑内糖生成或血脑屏障紊乱所致，与脑功能紊乱有关。血清素2A受体抗体和酪氨酸羟化酶抗体显示，人腹侧段和黑质的许多神经元被双重标记，表明人中脑多巴胺能神经元的活性可能在其起源核的水平上受到血清素2A受体的强烈调节。

Lectin histochemical technique clearly revealed the existence of senile plaque, neurofibrillary tangles, corpora amylacea, and three types of carbohydrate depositions, that is, spherical, vascular and stratiform type, in the human brain from patients with brain disorder. The spherical type deposits were detected in the molecular layer of the dentate gyrus of the hippocampal formation of patients with schizophrenia. The vascular and stratiform type which showed amorphous in the hematoxylin eosin staining were detected in the white matter region in the cerebrum and cerebellum of patients with brain disorder, such as dementia, Down's syndrome or brain edema. Dipeptidyl peptidase III (DPIII) and alanyl aminopeptidase (AP) were purified from human liver cytosol, and antibodies against these enzymes were produced. Antibody against AP histochemically reacted with neurons and glial cells, and could stain the above mentioned amorphous depoditions. The depositions newly detected in this project may be results from the disturbance of glycogenesis or blood brain barrier in the brain and relate to brain disorder. Antibodies against serotonin 2A receptor and tyrosine hydroxylase revealed that many neurons in the human ventral tegment area and substantia nigra are doubly labeled and suggested that activity of human midbrain dopaminergic neurons might be strongly regulated via serotonin 2A receptors at the level of their originating nuclei.