Experimental Periapical Lesion in Non-insulin Dependent Diabetic Rat (GK Rat).

本研究旨在观察蔗糖溶液对Goto&Kakizaki建立的自发性非胰岛素依赖型糖尿病大鼠(GK大鼠)根尖周病变的影响。GK大鼠20只，JCL大鼠(JCL大鼠)20只。所有动物均接受左侧下颌第一磨牙牙髓暴露治疗。然后将动物平均分为JCL大鼠(J-1、J-2)和GK大鼠(G-1、G-2)。J-1和G-1接受正常的实验室饮食和水，而J-2和G-2接受正常的实验室饮食和30%的蔗糖溶液。4组动物分别于牙髓暴露后2周和4周处死动物，进行组织学观察。血糖检测显示，G-1和G-2在4周和12周时的血糖值高于J-1和J-2。对下颌第一磨牙的牙髓组织(PT)和根尖周组织(PAT)进行组织学检查。此外，对PAT根尖周病变区域进行了组织计量学测量。组织学上，在整个实验期间，PAT的牙槽骨吸收在J-2组比G-2组严重。组织计量学研究显示，G-2组在2周和4周时根尖周病变面积明显大于J-2组(p&lt；0.01)。本研究结果提示，非胰岛素依赖型糖尿病的代谢状态可能促进了大鼠根尖周病变的发展。

The purpose of the present study was to investigate histologically and histometrically the effect of a sucrose solution on the periapical lesion of spontaneous non-insulin dependent diabetes mellitus rats (GK Rat) which were made by Goto ＆ Kakizaki. Twenty GK rats and 20 JCL Wistar rats (JCL Rat) were used in this study. All animals received a pulpal exposure treatment in the left mandibular first molar. Then, the animals were divided equally into two groups of JCL rats (J-1, J-2) and two groups of GK rats (G-1, G-2). J-1 and G-1 received a normal laboratory diet and water, while J-2 and G-2 received a normal laboratory diet and 30% sucrose solution. The animals of all 4 groups were sacrificed 2 and 4 weeks after pulpal exposure for histological observation. The blood glucose tests showed higher values at 4 and 12 weeks in G-1 and G-2 than in J-1 and J-2. Pulpal tissues (PT) and periapical tissues (PAT) of the mandibular first molar were examined histologically. Moreover, the area of the periapical lesion in PAT was measured histometrically. Histologically, the alveolar bone resorption in PAT was severer in J-2 than that in G-2 throughout the experimental period. The histometrical study revealed that the area of the periapical lesion was significantly larger in G-2 at 2 and 4 weeks than that in J-2 (p<0.01). The results of the present study indicate that the metabolic condition of non-insulin dependent diabetes mellitus may enhance the development of periapical lesions in rats.