Cytochemical and molecular biological study on the control mechanisms of differentiation and function of steroid hormone synthesizing cells.

(1)免疫组化研究犬前列腺腺上皮细胞中5α-还原酶的定位。抗雄激素药物治疗后，细胞的免疫反应性降低。(2)在大鼠脊髓中，肺型PGF合酶免疫定位于神经细胞和血管内皮细胞的树突和细胞体中。(3) 17β-羟基类固醇脱氢酶免疫定位于大鼠嗅球神经细胞。(4)为了获得人工抗体，我们制作了表达人VH的噬菌体文库。(5)未发现参与肾上腺皮质细胞分化的因子。(6)采用免疫细胞化学和酶组织化学方法研究了NO合成酶在老年大鼠和糖尿病大鼠盆腔神经节中的表达。结果表明，该酶在两大鼠神经节中的表达均降低，尤其是糖尿病大鼠。(7)采用生化和免疫细胞化学方法研究了围产儿大鼠胃饥饿素的表达。围产期大鼠血液中胃饥饿素水平较高。生长素产生的主要部位免疫定位于胎盘的合体滋养细胞。出生后，胃促生长素的产生部位是胃腺的内分泌细胞。

(1) Immunohistochemical studies localized 5α-reductase in the glandular epithelial cells of the canine prostate. After the treatment of an anti-androgenic drug, the immunoreactivity of the cells was decreased.(2) In the rat spinal cord, lung type PGF synthase was immunolocalized in the dendrites and cell bodies of nerve cells and vascular endothelial cells.(3) 17β-Hydroxysteroid dehydrogenase was immunolocalized in the nerve cells of the rat olfactory bulb.(4) In order to obtain an artificial antibody, we made a phage library expressing human VH.(5) We could not get factors involved in the differentiation of adrenal cortical cells.(6) The expression of NO synthase is studied in the major pelvic ganglia of aged and diabetic rats by means of immunocytochemistry and enzyme histochemistry. The results indicated that the expression of the enzyme was decreased in the ganglia of the both rats, especially in the diabetic one.(7) The expression of ghrelin was studied biochemically and immunocytochemically in the perinatal rats. The blood ghrelin level was high in the perinatal rats. The major site of the ghrelin production was immunolocalized in the syncytiotrophoblast of placenta. After birth, the site of ghrelin production was endocrine cells of the gastric glands.