Identification of Human facilitating cells

我们利用人/小鼠氙气移植模型寻找人促生细胞。在人骨髓CD8阳性细胞中，CD56阳性细胞占30 ~ 40%，CD161阳性细胞占15 ~ 20%。骨髓细胞分为CD8+CD56+， CD8+CD56 -， CD8+CD161+和CD8+CD161-使用FACS Vantage作为促进细胞的候选细胞。将总骨髓细胞、CD34+c-kit+细胞（干细胞）、CD8+促生细胞候选细胞和干细胞+促生细胞候选细胞分别移植到300只cGy辐照的NOD / SCID小鼠体内。移植8周后，处死小鼠，测定骨髓细胞中是否有人CD45阳性细胞。总骨髓细胞移植后CD45阳性细胞比例为1 ~ 7%，干细胞移植后CD45阳性细胞比例为3 ~ 10%，CD8阳性细胞移植后+促性细胞候选细胞比例为0%，干细胞+促性细胞候选细胞移植后CD45阳性细胞比例为3 ~ 18%。在促进细胞的四种候选物和促进细胞移植的候选物之间没有发现显著差异。异种移植的低移植效率被认为是导致阴性数据的原因，因此我们选择新生的NOD / SCID β2微球蛋白-/-小鼠作为受体。全骨髓细胞移植后，人CD45阳性细胞率达90%以上。使用新的氙气移植模型的研究仍在继续。我们研究了婴儿中性粒细胞减少症，并报道了一些新的信息。

We sought human facilitating cells using human / mouse xenon-transplantation model.Among human bone marrow CD8 positive cells, 30〜40% cells were positive for CD56 and 15 〜20% cells were CD161 positive. Bone marrow cells were sorted into CD8+CD56+, CD8+ CD56-, CD8+CD161+and CD8+CD161-using FACS Vantage as candidates for facilitating cells. Total bone marrow cells, CD34+c-kit+cells(stem cells), CD8+cells candidates for facilitating cells and stem cells plus candidates for facilitating cells were, respectively, transplanted into 300 cGy irradiated NOD / SCID mice. After 8 weeks in the transplantation, mice were sacrificed and the bone marrow cells were measured for human CD45 positive cells. Percentages of CD45 positive cells were 1 〜7% after total bone marrow cell transplantation, 3〜10% after stem cell transplantation, 0% after CD8 positive cell transplantation and candidates for facilitating cells, 3〜18% after stem cells plus candidates for facilitating cells transplantation. No significant differences were detected among the four candidates for facilitating cells plus candidates for facilitating cells transplantation. The low engrafting efficacy of the xenotransplantation was thought to cause the negative date, we then choose new born NOD / SCID β2microglobulin-/-mice as recipients. The engrafting efficacy was improved and over 90% of human CD45 positive cells were seen after total bone marrow cells transplantation. The study is still on going using the new xenon transplantation model.We have studied neutropenia of infancy and several new information were reported.