Development of rapid detection method of lymph node metastasis from prostate cancer

前列腺癌的主要治疗方法是前列腺癌根治术，虽然术前评估未发现明显的前列腺外转移，但部分患者术后出现复发或转移，临床需要高灵敏度的微转移检测，因此，我们建立了应用RT-PCR方法检测前列腺癌微转移的方法。该方法检测PSAmRNA，所需标本少，但反应时间长，不利于快速诊断。该方法灵敏度高，但不能定量。建立了毛细管内聚合酶链式反应快速诊断方法。新方法的反应时间仅为30分钟。建立了前列腺癌患者血清中前列腺癌细胞的定量检测方法，为前列腺癌微转移的临床研究奠定了基础。

The radical prostatectomy is the main therapy of prostate cancer.Though the evaluation before prostatectomy reveals no remarkable extra-prostatic metastasis, recurrence or metastasis occurs in some cases after prostatectomy.The high sensitive detection of micro-metastasis needs for clinically.Therefore, we developed detection method of micro-metastasis using RT-PCR method. This method detects PSAmRNA, needs a little specimen, but the duration of reaction is too long for rapid diagnosis. And this method has high sensitivity, but no quantity. We developed rapid diagnostic method using PCR in capillary. The duration of reaction of new method only 30 minutes. And we developed quantitative detection of prostatic cancer cell in the serum.These new method contributes clinical aspect of prostatic micro-metastasis.