Identification of genes conferring tolerance to submergence or waterlogging stress and generation of submergence-tolerant crops

快速的环境变化使植物受到各种非生物胁迫，对其生长发育产生不利影响。在日本，农作物（如大豆、大麦和小麦）在雨季经常因洪水和暴雨而缺氧。由于植物是无根的，它们对淹水或淹没压力的快速反应能力对适应干旱生存至关重要。为了鉴定能够赋予作物耐涝或耐淹性的基因，在本项目中，我们使用激活标记的突变文库和ems诱变的拟南芥突变文库筛选了耐淹性更强的突变体。最后，我们从310,000个激活标记系中选择了两个突变系，并确定了T-DNA插入边缘的核苷酸序列。以A90系为例，T-DNA被插入到At2g07682基因中，该基因来自转移的线粒体DNA。另一方面，在A96系的情况下，我们发现T-DNA插入到At5g39500基因中。目前，我们正在检查激活标记的鱼系是否真的对水下压力有更强的耐受性。

Rapid environmental changes subject plants to various abiotic stresses that adversely affect their growth and development. In Japan, crops (e.g., soybean, barley and wheat) are often deprived of oxygen by floods and heavy rainfall in rainy season. Because plants are sessile, their ability to respond quickly to the waterlogged or submergence stress is crucial for adaptation arid survival. To identify genes that can confer waterlogging or submergence tolerance to crops, in this project, we screened the mutants that show more tolerance to submergence using the activation-tagged mutant library and the EMS-mutagenized mutant library of Arabidopsis thaliana. Finally, we selected two mutant lines from 310,000 activation tagged lines and determined the nucleotide sequences at the border of T-DNA insertions. In the case of the A90 line, T-DNA was inserted into the At2g07682 gene, which was originated from the transferred mitochondrial DNA. On the other hand, in the case of the A96 line, we found that the T-DNA was inserted into the At5g39500 gene. Currently, we are checking whether the activation-tagged lines truly have more tolerant to submergence stress.