The study of molecular and gene analysis and bone metabolism-improving effect of propiomibacterium freudenreichii milk whey culture against osteoporosis.

为了了解免疫抑制剂与骨质疏松发生的关系，我们采用免疫抑制剂FK506注射裸鼠和野生型小鼠建立骨质疏松小鼠模型。野生型软骨成骨时骨小梁数量减少，软骨成骨时TRAP阳性破骨细胞数量明显增加。另一方面，裸鼠内软骨成骨后几乎没有骨吸收。提示免疫细胞通过免疫抑制剂参与骨质疏松的发生。我们发现瑞士奶酪发酵剂丙杆菌乳清培养物（PC）可以挽救我们模型中的骨质疏松症。在进一步的实验中，PC可以抑制破骨细胞的分化，预防骨质疏松。但很难简单地说，在我们的模型中，PC通过对破骨细胞的差异抑制来拯救骨质疏松症，因为破骨细胞有更多的分化和免疫系统参与。具体来看，我们得到了以下结果：a)用磁珠提取骨髓，添加CD4阳性t细胞或B220阳性b细胞的小鼠成骨样基质细胞和成骨细胞混合培养中，破骨细胞的数量明显增加。b) FACS结果显示免疫抑制剂可使模型脾和肠系膜淋巴结t细胞数量减少，b细胞数量增加；c) PC中含有萘醌类dna。基本骨架与维生素K2相同，是近年来骨量恢复的研究热点。dna与PC的结果相同，被认为是抑制骨吸收的一个因素。我们阐明了dna对骨量恢复的影响，以及免疫系统与破骨细胞的关系。此外，为了鉴定骨质疏松相关基因，我们用DNA芯片观察了骨髓中基因表达的改变。少

To see the relationship between immune cell and osteoporosis occurrence by immunosuppresant, we have established the osteoporosis mouse model using immunosuppresant (FK506) injected nude mouse and wild type mouse. The results of wild type were amount of trabecula bone under enchondral ossification was decreased, and number of osteoclast, TRAP positive, was obviously increased in enchondral ossification. On the other hand, there was almost no bone resorption under enchondral ossification in the nude mouse' s result. Those results suggest that immune cell contributes to the osteoporosis occurrence by the immunosuppresant. We have found propiomibacterium freudenreichii milk whey culture (PC), starter of Swiss cheese, could rescue osteoporosis in our model. In further experiment, PC could inhibit the differentiation of osteoclast and prevent osteoporosis. But it is hard to simply say that PC rescued osteoporosis in our model by differential inhibition of osteoclast, because of the osteocla … More st differentiation and immune system participation. To see the details, we have gotten the following results, a) The number of osteoclast was significantly increased in the mouse osteoblast-like stroma cell and osteoblast mixed culture with supplementation of CD4 positive T-cell or B220 positive B-cell, extracted from bone marrow with magnet beads. b) FACS result was the immunosuppresant decreased the number of T-cell and increased the number of B-cell in spleen and mesentericlymph node of our model, c) DHNA, contained in PC, has naphthoquinone. The basic skeleton is the same as vitamin K2, which is a research attention of bone mass recovery in recent years. DHNA made the same results as PC's and was identified as a factor of inhibitory effect of bone resorption.We elucidated the bone mass recovery with DHNA, and the relationship immune system and osteoclast. Furthermore, to identify osteoporosis-related gene, we have seen the alteration of gene expression in'bone marrow with DNA microarray. Less