Toward the reconstruction of genetics and breeding of bacterial blight resistance in ric

本研究进行了两类实验：1。基于图谱的Xa7基因克隆：将Xa7所在的区域缩小到供体DNA上128kb的区域。该区域有20种预测基因，其中不包括之前已知的R基因。对6个细分的DNA片段进行了转化，但由于用于转化的受体表现出一定的抗性，因此难以检查功能增益。由于IRRI可以处理大量T1植株进行接种试验，因此可以在IRRI进行互补试验。水稻野生种对白叶枯病的抗性及其基因定位：我们对大约300个野生种进行了评估。其中1 / 3对日本1和3两个小种表现出抗性，表明野生种显然是抗白叶枯病基因资源的重要材料。Xa11的映射研究发表在JARQ上。

Two kinds of experiments were conducted in this study ;1. Map-based cloning of Xa7 gene : The region in which Xa7 was located was narrowed down to the 128kb region on the donor DNA. There are 20 kinds of predicted genes in the region, where previously-known R genes are not included. Transformation of 6 subdivided DNA fragments was performed, but it was difficult to check gain-of function because the recipient used for transformation showed somewhat resistance. The experiment of complementation test would be done in IRRI, because IRRI can handle the many T1 plants for inoculation test.2. Bacterial blight resistance in wild species of rice and gene mapping of Bacterial blight resistance : We evaluated about 300 strains of wild species. One-third of them showed the resistance to two Japanese races 1 and 3, demonstrating that wild species are apparently important materials as gene resource for bacterial blight resistance. The mapping study of Xa11 was published in JARQ.