Analysis of extracellular distribution and cellular uptake system of thyroid-disrupting chemicals

为了阐明影响内分泌干扰物在靶细胞内生物学效应的因素，我们研究了2，4，6-三碘苯酚（TIP）和碘苯腈（IOX），有效的甲状腺激素干扰物，与虹鳟鱼，牛蛙，鸡，猪和大鼠血清蛋白的相互作用。[^<125>I]-化学物质与鳟鱼和牛蛙血清中的蛋白质结合较弱，与鸡、猪和大鼠血清样品中的蛋白质结合较强。[^<125>I]-化学物质与蝌蚪血清蛋白的弱相互作用加速了体外[^<125>I]-化学物质的细胞摄取。细胞对[^<125>I]-化合物的摄取表明存在苯酚特异性细胞摄取系统。脊椎动物中化学结合蛋白的分子和结合特性的差异，以及苯酚特异性细胞摄取系统将部分影响这些化学物质的细胞作用。

To clarify the factors that influence the biological effects of endocrine disrupting chemicals within target cells, we investigated the interaction of 2,4,6-triiodophenol (TIP) and ioxynil (IOX), potent thyroid hormone disrupting chemicals, with serum proteins from rainbow trout, bullfrog, chicken, pig, and rat. [^<125>I]-chemicals bound weakly to proteins in trout and bullfrog serum, and strongly to proteins in chicken, pig, and rat serum samples. A weak interaction of [^<125>I]-chemicals with tadpole serum proteins accelerated [^<125>I]-chemical cellular uptake in vitro. The cellular uptake of [^<125>I]-chemicals suggested the presence of phenol-specific cellular uptake system. The differences in the molecular and binding properties of chemical-binding proteins among vertebrates, and a phenol-specific cellular uptake system would affect in part the cellular actions of these chemicals.