Specific gene expression and its functional analysis in the maturing rat testis.

精子发生是一个复杂的过程，许多基因系统地表达。采用差异显示法筛选成熟大鼠睾丸中的4个基因。从其氨基酸序列推断，Hrasls5基因产物可能具有肿瘤抑制和精子发生的作用。RGD1303144基因在精母细胞中主要表达。结肠癌自身抗原基因Sdccag8也定位于7周龄大鼠睾丸的精母细胞，并在精母细胞中检测到其蛋白。Sdccag8可能在精母细胞向精母细胞的发育过程中起作用。原位杂交显示Ttc29主要在精母细胞中表达。FSH处理可上调Ttc29的表达。Ttc29编码轴突动力蛋白，轴突动力蛋白是精子鞭毛的组成部分。这些数据表明轴突动力蛋白的表达始于精母细胞，并受卵泡刺激素调节

Spermatogenesis is a complex process, and many genes are systematically expressed. We screened 4 genes in maturing rat testis using differential display method. Hrasls5 gene product might function as a tumor suppressor as well as in spermatogenesis, as deduced from its amino acid sequence. RGD1303144 gene was expressed dominantly in spermatocytes. Colon cancer autoantigen gene, Sdccag8, was also localized to the spermatocytes in 7-week-old rat testis, and its protein was detected in the spermatids. Sdccag8 may have a role in the development from spermatocyte to spermatid. In situ hybridization revealed that Ttc29 was expressed primarily in spermatocytes. Ttc29 expression was up-regulated by treatment with FSH. Ttc29 encodes axonemal dynein, which is a component of sperm flagella. These data indicate that axonemal dynein expression starts in spermatocytes and is regulated by FSH