Single-cell microscopy with stimulus and response device to analyze relaxation mechanism of ES cells fluctuation

在胚胎发育过程中提供稳定性的机制仍有待发现。为此，我们制作了一个系统来观察单细胞对培养基刺激的反应。该系统由四部分组成：胚胎干细胞，这是一个很好的早期发育模型，没有任何未知因素的培养基，一个特殊涂层的培养皿，使我们能够分离细胞而不聚集，以及延时显微镜。该系统使我们能够监测单个胚胎干细胞的生死、增殖和分化。单细胞水平观察胚胎干细胞的刺激反应将加深我们对发育稳定性的理解。

The mechanism that provides stability during embryonic development remains to be uncovered. To do this, we made a system to observe a single-cell reaction to culture medium stimulation. The system consists of four parts : ES cells, which are a good model for early development, a culture medium without any unknown factors, a specially coated culture dish that enabled us to dissociate cells without aggregation, and time-lapse microscopy. This system enabled us to monitor life-and-death, proliferation, and differentiation of a single ES cell. Single-cell level observation of stimulation response of ES cells will deepen our understanding of developmental stability.