Global transcriptome analysis of the pine wilt disease and application to the resistance breeding

日本黑松（Pinus thunbergii）极易感染由松材线虫（PWN; Bursaphelenchus xylophilus）引起的松材枯萎病。为了应对这种疾病，研究人员和树木育种者在以前的育种计划中选择了抗pwn的个体。为了了解其致病和耐药机制，我们使用LongSAGE（基因表达序列分析）和ODD（有序差异显示）分析来阐明其机制。SAGE转录组分析结果显示，接种PWN后，抗性和非抗性松树的致病相关蛋白、渗透蛋白、脂氧合酶、查尔酮合成酶均显著上调，真核翻译起始因子SUI1、翻译控制肿瘤蛋白和木葡聚糖内转糖基化酶均下调。另一方面，过氧化氢酶、二烯内酯水解酶家族蛋白仅在抗性松中有较高表达，致病相关蛋白和白花青素双加氧酶仅在非抗性松中有较高表达。这些差异表达的基因被认为反映了抗和非抗松树之间的一些差异。

The Japanese black pine(Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode(PWN ; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of pathopoiesis and resistance, the mechanisms were clarified using LongSAGE(serial analysis of gene expression) and ODD(ordered differential display) analyses. Especially, by the global transcriptome analysis using SAGE, it is indicated that pathogenesis-related proteins, osmotin, lipoxygenase, chalcone synthase were significantly up-regulated, and eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase were down-regulated by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, catalase, dienelactone hydrolase family protein were expressed at a higher level only in the resistant pine, pathogenesis-related proteins and leucoanthocyanidin dioxygenase were expressed only in the non-resistant pine. These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines.