Antimicrobial and osteoconductivity of titanium implant surface.

微生物在骨科和牙科植入物表面的初始黏附是触发生物膜形成的不可避免的事件。植入型医疗器械上生物膜的形成是导致潜在手术失败的主要并发症。当钛在氯化钠溶液中放电氧化时，既具有抗菌活性，又具有骨传导性。附着在抗菌钛上的微生物和细胞的生存能力仍然不确定。我们假设，薄薄的过氧化屏障将有效地摧毁附着的细菌，而附着的成骨细胞将是活的，因为这些细胞通过血清蛋白间接附着在表面。抗菌钛的功效似乎是基于过氧化，因为过氧化产物是与细菌细胞表面结构的破坏平行检测到的。抗菌钛的过氧化作用仅限于表面。成骨细胞表面的活性强烈依赖于血清蛋白的存在，而贴壁变形链球菌的活性不受血清蛋白的影响。因此，细菌和成骨细胞使用的贴壁系统的不同是其在抗菌钛表面活性的重要决定因素。

Initial adhesion of micro-organisms onto such orthopedic and dental implant surfaces is an unavoidable event that triggers biofilm formation. Biofilm formation on implanted medical devices is a major complication leading to potential surgical failure. When titanium is anodized by discharge in NaCl solution, both antimicrobial activity and osteoconductivity are conferred. The viability of adherent micro-organisms and cells on antimicrobial titanium remains uncertain. We hypothesized that a thin peroxidation barrier would efficiently destroy adherent bacteria, whereas adherent osteoblastic cells would be viable, since these cells adhere to the surface indirectly though serum proteins. The efficacy of antimicrobial titanium appears to be based on peroxidation, since peroxidation products were detected in parallel with the destruction of bacterial cell-surface structures. The peroxidation effect of antimicrobial titanium was confined to the surface within narrow limits. The viability of osteoblastic cells on the surface was strongly dependent on the presence of serum protein, whereas that of adherent Streptococcus mutans was not affected by the presence of serum proteins. Therefore, differences in the adherent systems used by bacteria and osteoblastic cells are important determinants of their viability on antimicrobial titanium.