Establishment of reproductive technology by centri-fusion method for porcine oocytes

本研究的目的是建立无需显微操作的生殖生物技术，而是通过融合猪卵母细胞离心后获得的卵浆片段。这些碎片由褐色和透明的碎片组成；前者含有大量的线粒体。棕色碎片重建的卵母细胞受精能力较好。将发育能力较低的卵母细胞与碎片融合后，其受精和发育能力均有所提高，两类碎片的受精和发育能力无明显差异。此外，受精卵母细胞片段与成熟卵母细胞的融合表现出卵母细胞活化能力。这些发现现在有望用于进一步改进技术。

The objective of the study is to establish reproductive biotechnologies without micromanipulation but by fusion of ooplasmic fragments obtained after centrifugations of porcine oocytes. The fragments were consisted of brownish and transparent ones; the former one contains a lot of mitochondria. The oocytes reconstructed from brownish fragments showed better fertilization ability. The oocytes showing low developmental ability were fused with the fragments, their fertilization and developmental abilities were improved, of which abilities were not different between two types of fragments. Furthermore, fusion of the fragments prepared from fertilized oocytes with the matured ones showed oocyte activation ability. These findings are now expected for further improvements of the technologies.