Molecular epidemiological and cytological studies on Plasmodium falciparum distributing in Southeast Asian continent or island areas

我们之前的研究结果表明，从缅甸和越南收集的恶性疟原虫野生分离株很容易适应体外培养，而从印度尼西亚岛屿收集的恶性疟原虫不适应体外培养。这些结果可能表明印度尼西亚野生分离株与分布在东南亚大陆的分离株有不容易适应体外培养的倾向。因此，开展这项研究是为了证实这些先前的发现，并对从东南亚大陆和印度尼西亚岛屿收集的野生分离株进行细胞学和分子流行病学比较。从印度尼西亚多个岛屿采集的20株野生分离株均不能再次适应体外培养；所有寄生虫在培养7 d内消失。另一方面，从越南和柬埔寨收集的7株野生分离株适合离体培养。特别是，从越南收集的分离物生长良好，形成了许多年轻的配子体。该菌株可以通过降低培养基中的葡萄糖水平诱导配子体的大量产生。将该分离物与一株印度尼西亚分离物进行了分子水平上的比较，采用Roche Genome Sequencer FLX+ System （Pyrosequence法）进行了比较，但尚未发现两者有任何差异。

Our previous findings showed that wild isolates of Plasmodium falciparum collected from Myanmar and Vietnam were easily adapted to in vitro culture, whereas those collected from Indonesian islands failed to be adapted. These results may suggest that Indonesian wild isolates has a tendency not to easily adapt to in vitro culture, differing from those distributing in continental SEA. Therefore, this study was conducted for confirmation of these previous findings and for cytological and molecular epidemiological comparisons between wild isolates collected from continental SEA or from Indonesian islands. All of 20 wild isolates collected from many Indonesian islands could not be adapted to in vitro culture again; all parasites disappeared within 7 days in culture. On the other hand, seven wild isolates collected from Vietnam and Cambodia were adapted to in vitro culture. Particularly, an isolate collected from Vietnam grew well, and it formed many young gametocytes. This isolates could be induced mass-production of gametocytes by decreasing glucose level in the culture medium. By using this isolate and an Indonesian isolate, comparison at molecular level was tried by the Roche Genome Sequencer FLX+ System (the Pyrosequence method), but any difference between them was not found yet.