New approach for the elucidation of the etiology of Kawasaki disease

我们收集了58例符合川崎病诊断标准并在我院住院的患者的咽拭子样本。将咽拭子细菌置于脑-心输注液体培养液中培养。使用DNA Mini Kit （QIAGEN）试剂盒提取细菌总DNA。以细菌总DNA为模板，对5个SAg基因-链球菌热原外毒素a型（SPE-A）、SPE-C、SPE-G、SPE-J和中毒性休克综合征毒素-1 （TSST -1）进行聚合酶链反应（PCR）。58例患者中检测到SPE-G基因7例，检测到SPE-J基因6例。58例患者中有1例同时检测到SPE-G和SPE-J基因。因此，在58例患者的12例（20.7%）咽拭子样本中检测到来自A群链球菌（GAS）的超抗原基因片段。这些结果是重复的，非常重要。然而，在所有检测到SPE-G和SPE-J基因的患者中均未检测到GAS。因此，任何一种细菌都具有这些超抗原基因是本研究的重点和主题。不幸的是，我们无法在这项研究中分离出细菌。

We collected the samples of throat swab from 58 patients who met the diagnostic criteria for Kawasaki disease and were admitted to our hospitals. Bacteria from throat swab were cultured in liquid culture solution (Brain-Heart-Infusion). W e extracted total DNA from those bacteria using DNA Mini Kit (QIAGEN). Using total DNA extracted from bacteria as a template, polymerase chain reaction (PCR) was performed for 5 SAg genes-streptococcal pyrogenic exotoxin type A(SPE-A), SPE-C, SPE-G, SPE-J, and toxic shock syndrome toxin-1 (TSST -1). SPE-G gene and SPE-J gene were detected in 7 and 6 of 58 patients, respectively. Both SPE-G gene and SPE-J gene were detected in one of 58 patients. Thus, gene fragments of superantigens derived from group A streptococcus (GAS) were detected in the samples of throat swabs from 12 of 58 patients (20.7%). These results were reproducted and very important. However, GAS was not detected from any patients in whom SPE-G and SPE-J gene were detected. Thus, it is very important point and the theme in this study that any kind of bacteria have these superantigen genes. Unfortunately, we could not isolate the bacteria in this study.