In vitro production of the Camel heavy chain antibody toward development of alternative antibody medicine

骆驼的单链抗体仅由重链组成。与标准的H2 L2抗体相比，单链抗体具有许多有利于抗体药物开发的化学特性，例如高水溶性和/或热稳定性。本研究利用染色体工程技术在细胞培养系统中生产骆驼重链抗体。我们已经成功地将含有完整抗体基因簇的骆驼染色体克隆到培养细胞中，然而，我们在体外没有检测到任何骆驼重链抗体，表明骆驼免疫系统与其他骆驼免疫系统不相容，尽管它们具有高度的序列相似性。

Camels have single-chain antibody consisting of only heavy chains. Comparing with standard H2L2 antibodies, single-chain antibody have many advantageous chemical features toward antibody-medicine development, such as high solubility in water and/or thermo-stability. In this study we employed chromosomal engineering to produce camel heavy chain antibody in cell culture system. We have successfully cloned the camel chromosome containing whole antibody-gene cluster into cultured cells, however, we could not detect any camel heavy chain antibody in vitro, suggesting incompatibility of camel immune system with others in spite of highly sequence similarity.