Molecular mechanism of rolling circle DNA replication of human papillomavirus

为了阐明人乳头瘤病毒DNA复制的机制，我们寻找影响病毒DNA在细胞内复制的因素。我们进行了ALPHA筛选，并确定了10个酪氨酸激酶结合HPV 16 E1。我们还确定了一个未知的激酶结合到E1之间的酪氨酸激酶底物肽磷酸化肽，并推测7激酶。将测试这些激酶与细胞中E1之间的相互作用以及激酶对HPV DNA复制的影响。

To elucidate the mechanism of DNA replication of human Papillomavirus, we searched for a factor that affects the virus DNA replication in cells. We performed ALPHA screening and identified ten tyrosine kinases that bind to HPV16 E1. We also identified peptides phosphorylated by an unknown kinase bound to E1 among a set of tyrosine kinase substrate peptides, and speculated seven kinases. Interaction between these kinases and E1 in cells and the effects of the kinases to HPV DNA replication will be tested.