Analysis of cellular signal propagation of local stimulation by cell recognizable micro-sphere

大量的研究表明，在一个群体中，细胞间的信号传递参与了细胞间的信号传递。为了分析信号在细胞集落中的传播机制，我们设计了局部刺激技术，利用e -钙粘蛋白固定微球或水凝胶在细胞片上给予局部机械刺激。我们发现，局部刺激e -cad球到MDCK细胞的外围，抑制了细胞集落的集体运动，并使ERK信号失活，使其传播到细胞集落。此外，我们发现，局部机械刺激细胞片诱导ERK信号传递到非刺激部分的细胞，这表明参与肌动蛋白网络的机械平衡是参与细胞集体运动的信号传播的换能器之一。

Numerous studies have shown that there are signal propagation between cells in a colony involved in the intercellular signaling. To analyze mechanism of the signaling propagation in the cell colony, we designed local stimulation technique using E-cadherin-immobilized micro-spheres or hydrogel, which gives local mechanical stimulation in a cell-sheet. We found that the local stimulation of E-cad-spheres to the periphery of the MDCK cell inhibit collective motion of the cell colony and inactivate ERK signaling, which propagated into the cell colony. Also, we found that local mechanical stimulation to the cell sheet induced ERK signaling to cells in a non-stimulated section suggested mechanical balance involved in actin network is one the transducer of signal propagation involved in collective cellular motion.