Involvement of microflora-induced Th17 cells in psoriasis

比较无菌小鼠和对照SPF小鼠的皮肤表型。与SPF小鼠相比，无菌小鼠的表皮萎缩。角质形成细胞分化的标记物，如角蛋白进行组织学染色。接下来，我们研究了病原体是否会触发牛皮癣的发展。金黄色葡萄球菌和表皮葡萄球菌经热灭活后加入培养的角质形成细胞中。诱导产生细胞因子和抗菌肽。咪喹莫特是一种天然免疫激活剂，用于银屑病皮损的形成。刮去小鼠背部皮肤，局部涂抹咪喹莫特，连续5天。涂药一周后，皮肤上出现红斑和鳞片。组织学分析证实了银屑病的发生。

Skin phenotypes of germ-free mice were compared with those of control SPF mice. The epidermis of germ-free mice was atrophic compared to SPF mice. Markers for keratinocyte differentiation such as keratins were histologically stained. Next, we studied whether pathogens can be a trigger for the development of psoriasis. S. aureus and S. epidermidis were heat-inactivated and added to the cultured keratinocytes. Cytokines and anti-microbial peptides were induced. Imiquimod, an activator of innate immunity, was used for the development of psoriatic skin lesions. Back skin of the mice was shaved and imiquimod was topically applied for the five consecutive days. One week after the application, erythema and scales appeared on the skin. Histological analyses confirmed the development of psoriasis.