Analysis of function of glucocorticoid receptor expressed in NPY neurons

体重和白色脂肪组织的重量显着减少在AgRP神经元特异性敲除的糖皮质激素受体小鼠相比，野生型小鼠，当他们受到高脂肪饮食。棕色脂肪组织中UCP1 mRNA的表达水平在基因敲除小鼠中显著高于野生型小鼠。另一方面，毒胡萝卜素，ER应激，增加磷酸化的p65和衰减的AgRP和NPY mRNA的表达刺激地塞米松在野生型小鼠下丘脑培养。p65抑制剂可拮抗毒胡萝卜素对地塞米松刺激的AgRP和NPY mRNA表达的影响。

Body weight and white adipose tissues weight were significantly less in AgRP neuron-specific knockout of glucocorticoid receptor mice compared to wild-type mice when they were subjected to a high fat diet. The expression levels of UCP1 mRNA in the brown adipose tissues were significantly higher in the knockout mice than in wild-type mice. On the other hand, thapsigargin, an ER stressor, increased phosphorylation of p65 and attenuated expression of AgRP and NPY mRNA stimulated by dexamethasone in hypothalamic cultures of wild-type mice. Furthermore, it is demonstrated that inhibitor of p65 cancelled the effects of thapsigargin on the AgRP and NPY mRNA expression stimulated by dexamethasone.