Immunohistochemical study of the glial function on the plastisity of medullary notiception

为探讨神经胶质功能对三叉神经脊髓核可塑性的影响，对大鼠延髓水平切片进行免疫组织化学染色。首先对石蜡切片进行免疫酶技术处理。之后，在自由浮子切片上进行免疫荧光，以获得更有效和有价值的结果。在深度麻醉下用多聚甲醛灌注固定后，取新生大鼠延髓。取延髓50 ~ 100 μ m厚的未冷冻水平切片，用荧光抗体法染色。三叉神经脊髓核内可见小胶质细胞（IbaI）或星形胶质细胞（GFAP）的高强度荧光信号。因此，该方法对三叉神经脊髓核神经胶质功能的研究是有用的。

To investigate the role of the glial function on the plastisity of the spinal trigeminal nucleus, immunohistochemical staining were performed on the horizontal slices of the rat medulla oblongata. At first, immunoenzyme technique were performed on the paraffin sections. Thereafter, immunofluorescence on the free-float sections were performed to obtain more efficient and valued results. After perfusion fixation with paraformaldehyde under deep anesthesia, the medulla oblongata were extirpated from neonatal rats. Thereafter, 50-100 micron-thick unflozen holizontal slices of the medulla oblongata were made, then stained with fluorescent antibody method. As a result, high intensity fluorescence signals of microglia(IbaI) or astrosyte(GFAP) were observed in the spinal trigeminal nucleus. Thus, the usefulness of this method for the investigation of the glial functions in the spinal trigeminal nucleus was suggested.