Construction and analysis of the evaluation system of environmental chemical substances on longevity using a longevity gene as an index of assessment

为了构建新的环境污染物评价体系，我们利用PC12细胞系统检测了化学污染物对sirtuin家族表达的影响。结果表明，邻苯二甲酸二乙酯(一种潜在的内分泌干扰物和促进细胞凋亡的因素)、铜或过氧化氢处理PC12细胞后，Sirt 1的表达显著上调。此外，还观察到营养不良孕鼠的子代Sirt1和SIRT2表达下降的趋势。结果表明，Sirt1和SIRT2的测定是评价生物体内化学物质的有用工具。

To construct a new evaluation system for environmental contaminants, we have examined effects of chemical contaminants on expression of sirtuin family using PC12 cell system. As results, expression of Sirt 1 was significantly up-regulated by treatment of diethyl phthalate (one of the potential endocrine disrupters and enhance element of apoptosis in the cell), copper or hydrogen peroxide in PC12 cells. In addition, the tendency that expression of Sirt1 and Sirt2 was decreased in the rat offspring from undernourished pregnant mothers was observed. From the results, it was concluded that measurement of Sirt1 and Sirt2 were useful tool for evaluation of chemical substances on organism.