Identification of stromal cells that have suppressive role againstpancreatic cancer cells and development of new anticancer therapy

本研究的目的是鉴定抑制胰腺癌细胞的基质细胞亚群。对胰腺导管腺癌（PDAC）来源的胰腺星状细胞（PSCs）进行表面标志物分析，并对CD 271 + PSCs进行定量RT-PCR分析，结果显示与胰腺癌细胞共培养后，PSCs中CD 271 mRNA表达增加。CD 271 mRNA表达水平在短暂升高后又下降。PDAC免疫组化显示间质CD 271高表达与良好的预后相关。这些结果表明，CD 271 + PSCs出现在胰腺癌发生的早期阶段，并通过肿瘤-间质相互作用对胰腺癌细胞具有抑制作用

The aim of the present study was to identify subpopulation of stromal cells that suppresspancreatic cancer cells. We analyzed surface marker of pancreatic stellate cells(PSCs) derived from human pancreatic ductal adenocarcinoma(PDAC), and characterized CD271+ PSCs.Quantitative RT-PCR analyses revealed that CD271 mRNA expression was increased in PSCs after coculture with pancreatic cancer cells. However, the level of CD271 mRNA expressionsubsequently decreased after the transient increase. Furthermore, CD271 mRNA expression was decreased in PSCs migrating toward pancreatic cancer cells through Matrigel.Immunohistochemistry of PDAC revealed stromal CD271 high expression was associated with a good prognosis. These findings suggest that CD271+ PSCs appear at the early stage ofpancreatic carcinogenesis and have suppressive role against pancreatic cancer cells through cancer-stromal interaction