Anaerobic spores (C. perfringens) as a possible new fecal source tracking indicator of human fecal pollution.

产气荚膜梭菌比目前传统的粪便指示剂在各种水环境中都能存活。因此，产气荚膜梭菌被认为是粪便污染的合适指示物。本研究通过CPE阳性产气荚膜梭菌的分布描述了产气荚膜梭菌作为一种有用的人类粪便污染源跟踪指标的新可能性。为评价产气荚膜梭菌溯源指标的有效性，对污水和畜禽粪便样品中产气荚膜梭菌孢子的分布及毒素类型进行了分析。用多重聚合酶链式反应从牛和猪的污水、污水和粪便中分离出产气荚膜梭菌孢子。从人类污水和流出物中几乎所有的分离株都被归类为A型，CPE阳性的产气荚膜梭菌占30%。而从家畜样品中只有1个黄牛分离株CPE基因阳性。CPE阳性产气荚膜梭菌的分布可作为人类粪便污染的潜在来源追踪指标。

Clostridium perfringens survive in various aquatic environments than current traditional fecal indicator. Therefore, C. perfringens considered as suitable indicator for fecal pollution. This research described that the new possibility of C. perfringens as a useful source tracking indicator of human fecal pollution using distributions of cpe positive C. perfringens. To evaluate the efficacy of source tracking indicator of C. perfringens, distributions of C. perfringens spores and there toxin type in sewage and livestock fecal samples were analyzed. C. perfringens spores were isolated from sewage, effluents and fecal samples of cattle and pig by multiplex PCR. Almost all isolates were classified type A. From human sewage and effluents, cpe positive C. perfringens were detected at 30%. Whereas only 1 cattle isolate were positive cpe gene from livestock samples. Distributions of cpe positive C. perfringens were important as a potential source tracking indicator for human fecal pollution.