Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis

这项研究的问题是，未培养的脂肪来源的再生细胞(ADRC)可以很容易地从自体脂肪组织中分离出来，并含有几种类型的干细胞和再生细胞，在牵张成骨中是否促进骨形成。将60只大鼠随机分为3组。牵张结束后即刻向牵张骨痂内注入生理盐水(对照组)、I型胶原胶或ADRC+I型胶原凝胶混合物(ADRC组)，ADRC组牵张骨痂注射后6周骨密度较对照组增加46%。注射后6周，ADRC组骨折强度较对照组提高66%。实时定量聚合酶链式反应显示ADRC组牵张骨痂中BMP-2、VEGFA和SDF-1的表达水平较高。新生骨中的细胞标记显示，ADRCs在注射后3周分化为骨组织。

The question of this study was whether uncultured adipose-derived regenerative cells(ADRCs), which can easily be isolated from autologous adipose tissue and contain several types of stem and regenerative cells, promote bone formation in distraction osteogenesis. Sixty rats were randomly assigned to 3 groups. Physiological saline (control group), Type I collagen gel, or a mixture of ADRC and Type I collagen gel (ADRC group) was injected into the distracted callus immediately after distraction termination.The bone density of the distracted callus in the ADRC group increased by 46% compared with the control group at 6 weeks after injection. The fracture strength in the ADRC group increased by 66% compared with the control group at 6 weeks after injection. Real-time PCR of the distracted callus from the ADRC group had higher levels of BMP-2, VEGF A, and SDF-1. Cell labeling in the newly formed bone showed the ADRCs differentiated into osseous tissue at 3 weeks after injection.