Preliminary study on endogenous disease diagnosis by chemical analysis in hair

有些疾病是由代谢紊乱引起的。因此，生化信息将在疾病中发挥重要作用。目前还没有足够的报告来诊断这些疾病的生化信息。为了获得生化信息，尿液和血液在临床诊断中是有用的。然而，样本应取自受饮食控制的患者进行检测。所以，死后的样本不适合这个目的。同时，据说毛发药物分析是证明慢性药物治疗（或使用）的有效工具。收集的毛发可以很容易地在环境条件下保存。我们的研究项目是根据头发药物测试计划的，如果过量的生物源化合物被头发保留。1. Extrolut [○！R]柱用于从毛发标本中获取化学信息。该方法灵敏度高，能检出低浓度的胺类、氨基酚类化合物。2.在头发中检测到3种化合物。在其中2份（未鉴别）独立标本中未定量观察到差异。酪胺（其中之一）的检测定量分为两个不同的组。3.除腐败标本外，约30%的标本中存在高水平的酪胺。从安非他明滥用者、心脏病患者和精神分裂症患者中获得的毛发标本相对较多。通过本项目的工作，指出了头发分析在解决迄今为止被掩盖的代谢紊乱方面的未来效用。

Some disease is caused by metabolic disorders. Therefore, biochemical information would play an important role for disorders. There are no adequate reports to diagnose those disorders by biochemical information. To obtain biochemical information, urine and blood are useful in clinical diagnosis. However, the samples should be taken from patients who are diet-controlled for the test. So that, postmortem samples are not suitable for the purpose. Meanwhile, it is said that hair drug analysis is an effective tool for demonstrating chronic drug therapy (or use). Hair collected can be easily preserved under ambient conditions. Our research project was planned based on the hair drug test, if excess biogenic compounds were retained by hair. 1. Gas chromatographic-mass spectrometric analysis together with extractive heptafluoro-n-butyrylation on Extrelut【○!R】 column was employed for acquiring chemical information from hair specimens. In this method, amines, aminophenols were sensitively detected in low level. 2. There were three compounds remarkably detected in hair. No difference among independent, specimens was quantitatively observed in two of them (unidentified). The detections of tyramin (one of them) were quantitatively divided into two different groups. 3. High levels of tyramine in hair were observed in about 30% of total specimens excepting putrefied specimens. Relatively numbers of hair specimens obtained from amphetamine-abusers, heart-disease holders and schizophrenic were observed. By this project work, the future utility of hair analysis is pointed out in resolving metabolic disorders that have so far been veiled.